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The symbiotic interaction of the dinoflagellate Alexandrium ostenfeldii with the diatom Pseudostaurosira serrata is well known for its ability to survive and sequester toxic alkenones under increasing saline conditions. However, it is unknown if this ability generalizes to other dinoflagellate-diatom symbioses and whether the capacity of A. ostenfeldii to sequestrate alkenones differs between freshwater and brackish water. We measured the growth of P. serrata in the presence of A. ostenfeldii in salinities ranging from freshwater to 30 psu. Our results showed that P. serrata grew at all salinities tested except at 30 psu in which the diatom flagellated but produced no alkenones. In comparison to growth in pure seawater, growth in co-culture with A. ostenfeldii was almost unaffected by salinity up to 20 psu, and then a reduction in growth occurred, although still above that of pure seawater. In contrast to growth in freshwater and seawater, P. serrata flagellated and sequestered alkenones at salinities ranging from 10 to 15 psu in co-culture with A. ostenfeldii. At salinities between 15 and 20 psu, P. serrata flagellated at low cell densities, but at higher cell densities flagellation ceased and alkenones were produced. Thus, flagellation and sequestration of alkenones are not only characteristic of A. ostenfeldii-P. serrata symbiosis in freshwater, but also in brackish conditions.1. Field of the Invention
The invention concerns a method and a device for the clocking of a memory.
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